《植物生理学报》 2009, 45(11): 1101-1103

胡杨雌花离体培养中的不定根诱导和植株再生体系的建立

周燕^1,2, 高自旸³, 高述民^1,*, 李凤兰¹

1 北京林业大学生物科学与技术学院, 北京100083; 2 北京市园林科学研究所, 北京100102; 3 北京交通大学电气工程学院, 北京100044

通信作者：高述民；E-mail: gsm689@sohu.com；Tel: 010-62338717

摘　要：

正交实验的方差和极差分析表明, 胡杨雌花诱导的愈伤组织和芽的效果明显优于雄花; 雌花诱导愈伤组织的最适培养基是B5添加1.0 mg·L-1 6-BA和1.0 mg·L-1 NAA, 诱导芽的最适培养基为MS+0.5 mg·L-1 6-BA+0.2 mg·L-1 NAA, 诱导根的最适培养基为1/2MS+0.5 mg·L-1 IBA

关键词：胡杨; 雌花; 离体; 愈伤组织; 不定根

收稿：2009-08-07   修定：2009-10-09

资助：国家自然科学基金(30671709) 和北京林业大学林木、花卉遗传育种教育部重点实验室开放基金(05-05)。

Plant Regeneration and Adventitious Root Induction in vitro from Female Flower of Populus euphratica Oliv.

ZHOU Yan^1,2, GAO Zi-Yang³, GAO Shu-Min^1,*, LI Feng-Lan¹

¹College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China; ²Beijing Institute of Landscape Gardening, Beijing 100102, China; ³School of Electrical Engineering, Beijing Jiaotong University, Beijing 100044, China

Corresponding author: GAO Shu-Min; E-mail: gsm689@sohu.com; Tel: 010-62338717

Abstract:

The orthogonal design exhibited the greatest impact factor for callus and buds induction of inflorescence types, and the results showed female inflorescence is better than male inflorescence. The optimum medium for callus induction of female inflorescence is B5+1.0 mg·L^-1 6-BA+1.0 mg·L^-1 NAA. The optimum medium of buds induced is MS+0.5 mg·L^-1 6-BA+0.2 mg·L^-1 NAA, and 1/2MS+IBA 0.5 mg·L^-1 for root induction.

Key words: Populus euphratica Oliv.; female flower; in vitro; callus; adventitious root